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"Molecular cloning of a gene, DHS1, which complements a drug-hypersensitive mutation of the yeast Saccharomyces cerevisiae." |
Lee YS, Shimizu J, Yoda K, Yamasaki M |
Published Jan. 1, 1994 in Biosci Biotechnol Biochem volume 58 .
Pubmed ID: 7764548
Abstract:
| We isolated a Saccharomyces cerevisiae mutant, CH108, which shows hypersensitivity to cycloheximide, bleomycin, actinomycin D, 5-fluorouracil, and several other antibiotics. The mutant cells showed irregular shapes with swelling and were also hypersensitive to Zymolyase digestion. The mutant cell wall was swelled and twice as thick as the wild type cell wall. These defects were completely reversed by introduction of a genomic clone on a single-copy vector. Restriction analysis and sequencing showed that the responsible gene, DHS1, encodes a novel hydrophilic polypeptide of 483 amino acids. |
This publication refers to following REPAIRtoire entries:
| Proteins |
Last modification of this entry: Oct. 6, 2010
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