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"A simple, three-step procedure for the large scale purification of DNA ligase from a hybrid lambda lysogen constructed in vitro." |
Panasenko SM, Alazard RJ, Lehman IR |
Published July 10, 1978 in J Biol Chem volume 253 .
Pubmed ID: 350879
Abstract:
| A simple three-step procedure for the large scale purification of DNA ligase has been developed. THe source of enzyme is a strain of Escherichia coli with a hybrid lambda prophage constructed in vitro that bears the ligase overproducing gene lop 11 lig+ (Panasenko, S., Cameron, J., Davis, R. W., and Lehman, I. R. (1977) Science 196, 188-189). The procedure yields homogeneous enzyme in approximately 40% yield. |
This publication refers to following REPAIRtoire entries:
| Genes |
Last modification of this entry: Oct. 6, 2010
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