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"Effect of chloramphenicol and the recB gene product on DNA metabolism in Escherichia coli K12 strains defective in DNA ligase." |
Morse LS, Beck LA, Pauling C |
Published Aug. 10, 1976 in Mol Gen Genet volume 147 .
Pubmed ID: 785229
Abstract:
| We have examined DNA strand breakage, DNA degradation, and the rate of DNA synthesis in lig and lig-recB strains of Escherichia coli K12 incubated in the presence and absence of 3 mug/ml chloramphenicol. Substantial DNA strand breakage and DNA degradation is observed in the lig strain upon growth at 40 degrees C; however, such strand breakage and DNA degradation is not observed in th lig-recB strainl Incubation of the lig strain at 40 degrees C in the presence of 3 mug/ml chloramphenicol reduces the amount of DNA strand breakage and DNA degradation to the level observed in the lig-recB strain. Together, these results demonstrate that exonuclease V (the recBC gene product) is responsible for the increased DNA degradation associated with DNA ligase deficiency. |
This publication refers to following REPAIRtoire entries:
| Genes |
Last modification of this entry: Oct. 6, 2010
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