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"Escherichia coli dnaT gene function is required for pBR322 plasmid replication but not for R1 plasmid replication."

Masai H, Arai K



Published June 1, 1989 in J Bacteriol volume 171 .

Pubmed ID: 2656633

Abstract:
Plasmid pBR322 was unable to replicate in a temperature-sensitive dnaT1 strain at a nonpermissive temperature, whereas a pBR322-derived plasmid carrying the wild-type dnaT+ gene was able to replicate under the same conditions. In contrast to pBR322, plasmid R1 could replicate in the dnaT1 strain at a nonpermissive temperature. In keeping with this finding, in vitro replication of plasmid R1 did not require DnaT protein.


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Last modification of this entry: Oct. 6, 2010

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