"AlkB restores the biological function of mRNA and tRNA inactivated by
Ougland R, Zhang CM, Liiv A, Johansen RF, Seeberg E, Hou YM, Remme J, Falnes PO
Deleterious 1-methyladenine (1-meA) and 3-methylcytosine (3-meC) lesions
are introduced into nucleic acids by methylating agents. It was recently
demonstrated that the E. coli AlkB protein and a human homolog, hABH3, can
demethylate these lesions both in DNA and RNA. To elucidate the biological
significance of the RNA repair, we have tested whether such repair can
rescue the function of chemically methylated RNA. We demonstrate that a
methylation-induced block in translation of an mRNA can be readily
relieved by treatment with AlkB and hABH3 prior to translation.
Furthermore, we show that chemical methylation of tRNAPhe inhibits
aminoacylation and translation, but that the inhibition can be reversed by
AlkB and hABH3. AlkB-mediated repair of 1-meA in tRNA was also observed in
E. coli in vivo. Our data demonstrate that AlkB proteins can mediate
functional recovery of RNA exposed to methylation damage, supporting the
notion that RNA repair is important.
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Last modification of this entry: Sept. 3, 2009
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