Protein FULL name:
UvrABC system protein B, Excinuclease ABC subunit B.,
Protein SHORT name:
UvrB (Escherichia coli strain K-12 substr. MG1655) is product of expression of
UvrB is involved in:
DDS in Escherichia coli strain K-12 substr. MG1655
NER in Escherichia coli strain K-12 substr. MG1655
FUNCTION: The UvrABC repair system catalyzes the recognition and
processing of DNA lesions. A damage recognition complex composed
of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon
binding of the uvrA(2)B(2) complex to a putative damaged site, the
DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP
binding by uvrB and probably causes local melting of the DNA
helix, facilitating insertion of uvrB beta-hairpin between the DNA
strands. Then uvrB probes one DNA strand for the presence of a
lesion. If a lesion is found the uvrA subunits dissociate and the
uvrB-DNA preincision complex is formed. This complex is
subsequently bound by uvrC and the second uvrB is released. If no
lesion is found, the DNA wraps around the other uvrB subunit that
will check the other stand for damage.
SUBUNIT: Forms a heterotetramer with uvrA during the search for
lesions. Interacts with uvrC in an incision complex.
SUBCELLULAR LOCATION: Cytoplasm.
DOMAIN: The beta-hairpin motif is involved in DNA binding.
MISCELLANEOUS: According to PubMed:3515321, a cleaved form of the
protein was observed that resulted from the removal of about 40
amino acids from the C-terminus of the protein. The exact cleavage
site being unknown, it was proposed to be between Lys-630 and Ala-
631. There was no indication that cleavage occured in vivo and
therefore it is not known if it has any physiological
SIMILARITY: Belongs to the uvrB family.
SIMILARITY: Contains 1 helicase ATP-binding domain.
SIMILARITY: Contains 1 helicase C-terminal domain.
SIMILARITY: Contains 1 UVR domain.
Links to other databases:
Sequences of the E. coli uvrB gene and protein.
||Arikan E, Kulkarni MS, Thomas DC, Sancar A.
Nucleic Acids Res.
March 25, 1986
Structure of the uvrB gene of Escherichia coli. Homology with other DNA repair enzymes and characterization of the uvrB5 mutation.
||Backendorf C, Spaink H, Barbeiro AP, van de Putte P
Nucleic Acids Res
April 11, 1986
A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.
||Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T, et al.
June 1, 1996
The complete genome sequence of Escherichia coli K-12.
||Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y
Sept. 5, 1997
NMR assignments and secondary structure of the UvrC binding domain of UvrB.
||Alexandrovich A, Sanderson MR, Moolenaar GF, Goosen N, Lane AN
May 21, 1999
Crystal structure of Escherichia coli UvrB C-terminal domain, and a model for UvrB-uvrC interaction.
||Sohi M, Alexandrovich A, Moolenaar G, Visse R, Goosen N, Vernede X, Fontecilla-Camps JC, Champness J, Sanderson MR
Feb. 14, 2000
Clue to damage recognition by UvrB: residues in the beta-hairpin structure prevent binding to non-damaged DNA.
||Moolenaar GF, Hoglund L, Goosen N
Nov. 1, 2001
The presence of two UvrB subunits in the UvrAB complex ensures damage detection in both DNA strands.
||Verhoeven EE, Wyman C, Moolenaar GF, Goosen N
Aug. 1, 2002
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.
||Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T
Mol Syst Biol
Jan. 1, 2006
Last modification of this entry: Oct. 19, 2010.
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