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Protein FULL name:
5-formyluracil/5-hydroxymethyluracil DNA glycosylase [Escherichia coli str. K-12 substr. MG1655].
Nei (Escherichia coli strain K-12 substr. MG1655) is product of expression of nei gene.
Nei is involved in:
BER in Escherichia coli strain K-12 substr. MG1655
FUNCTION: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'- phosphates.
CATALYTIC ACTIVITY: Removes damaged bases from DNA, leaving an abasic site.
CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
COFACTOR: Binds 1 zinc ion per subunit.
SIMILARITY: Belongs to the FPG family.
SIMILARITY: Contains 1 FPG-type zinc finger.
Links to other databases:
Last modification of this entry: Oct. 15, 2010.
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