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Protein FULL name:
Regulatory protein Ada, Regulatory protein of adaptative response, Contains: Methylated-DNA-protein-cysteine methyltransferase, O-6-methylguanine-DNA alkyltransferase.,
Ada (Escherichia coli strain K-12 substr. MG1655) is product of expression of ada gene.
Ada is involved in:
DRR in Escherichia coli strain K-12 substr. MG1655
FUNCTION: Repair of alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Can also repair O-4-methylthymine.
FUNCTION: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB.
CATALYTIC ACTIVITY: DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L- cysteine.
COFACTOR: Binds 1 zinc ion per subunit.
MISCELLANEOUS: This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocytalyst that acts repeatedly and over many reaction cycles.
SIMILARITY: In the C-terminal section; belongs to the MGMT family.
SIMILARITY: Contains 1 HTH araC/xylS-type DNA-binding domain.
Links to other databases:
Ada (Escherichia coli strain K-12 substr. MG1655) is able to recognize following damages:
Last modification of this entry: Nov. 11, 2010.
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