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Heteroduplex DNA with removed mismatch (from 5')
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Reaction | Previous state | Following state |
5'-3' exonuclease digests erroneous strand some distance beyond mismatch in Escherichia coli strain K-12 substr. MG1655 | Heteroduplex DNA with digested erroneous strand from 5' in Escherichia coli strain K-12 substr. MG1655 | Heteroduplex DNA with removed mismatch (from 5') in Escherichia coli strain K-12 substr. MG1655 |
Reaction | Previous state | Following state |
DNA polymerase III resynthesizes DNA (5' directed EcMMR) in Escherichia coli strain K-12 substr. MG1655 | Heteroduplex DNA with removed mismatch (from 5') in Escherichia coli strain K-12 substr. MG1655 | DNA strand being resythesized by DNA polymerase III (5' nick) in Escherichia coli strain K-12 substr. MG1655 |