|
![]() Welcome! Click here to login or here to register. |
||||||||||||||||||||||||||||||||||||||||
|
Homoduplex DNA with Dam methylase bound to GATC sites
|
||||||||||||||||||||||||||||||||||||||||
| Reaction | Previous state | Following state |
| Dam binds GATC sites (3' directed EcMMR) in Escherichia coli strain K-12 substr. MG1655 | Heteroduplex DNA sealed by DNA ligase (3' nick) in Escherichia coli strain K-12 substr. MG1655 | Homoduplex DNA with Dam methylase bound to GATC sites in Escherichia coli strain K-12 substr. MG1655 |
| Dam binds GATC sites (5' directed EcMMR) in Escherichia coli strain K-12 substr. MG1655 | Homoduplex DNA sealed by DNA ligase (5' nick) in Escherichia coli strain K-12 substr. MG1655 | Homoduplex DNA with Dam methylase bound to GATC sites in Escherichia coli strain K-12 substr. MG1655 |
| Reaction | Previous state | Following state |
| Dam methylase methylates GATC sites in Escherichia coli strain K-12 substr. MG1655 | Homoduplex DNA with Dam methylase bound to GATC sites in Escherichia coli strain K-12 substr. MG1655 | DNA with fully methylated GATC sites in Escherichia coli strain K-12 substr. MG1655 |
|
|
|
|