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"Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene." |
Dong H, Lin W, Zhang CK, Xiong H, Fu G, Jin WR, Chen R, Chen Z, Qi ZT, Huang GM |
Published Jan. 21, 2001 in Gene volume 264 .
Pubmed ID: 11250073
Abstract:
| Chromatin assembly factor-1 (CAF-1) plays essential roles in eukaryotic chromatin assembly during DNA replication (Smith and Stillman, 1989. Cell 58, 15-25), (Krude, 1999. Eur. J. Biochem. 263, 1-5). Its p150 subunit, involved in interaction with histone H3 and H4, is critical to the CAF-1 nucleosome assembly activity. In this study, we sequenced a 96-kb genomic DNA region that includes a 42.8-kb CAF-1 p150 subunit gene (CHAF1A), and a 41.1-kb EEN gene. A scripted bioinformatics analysis pipeline (research agent) has been set up to annotate the BAC sequence with a set of integrated algorithms. The CAF-1 p150 subunit gene contains 15 exons and 14 introns. The promoter region is characterized by deletional analyses, revealing a potential repressor. Tissue-correlated alternative splicing forms of the transcript was initially identified by EST clustering analysis, then confirmed by RT-PCR which resulted more splicing forms than computational prediction. |
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Last modification of this entry: Oct. 6, 2010
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