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"Isolation, sequencing and overexpression of the gene encoding the theta subunit of DNA polymerase III holoenzyme." |
Carter JR, Franden MA, Aebersold R, Kim DR, McHenry CS |
Published July 11, 1993 in Nucleic Acids Res volume 21 .
Pubmed ID: 8341603
Abstract:
| The gene encoding the theta subunit of DNA polymerase III holoenzyme, designated holE, was isolated using a strategy in which peptide sequence was used to derive a DNA hybridization probe. Sequencing of the gene, which maps to 41.43 centisomes of the chromosome, revealed a 76-codon open reading frame predicted to produce a protein of 8,846 Da. When placed in a tac promoter expression vector, the open reading frame directed expression of a protein, that comigrated with authentic theta subunit from purified holoenzyme, to 6% of total soluble protein. |
This publication refers to following REPAIRtoire entries:
| Genes | |
| Proteins |
Last modification of this entry: Oct. 6, 2010
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