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"Product-assisted catalysis in base-excision DNA repair."

Fromme JC, Bruner SD, Yang W, Karplus M, Verdine GL



Published March 1, 2003 in Nat Struct Biol volume 10 .

Pubmed ID: 12592398

Abstract:
Most spontaneous damage to bases in DNA is corrected through the action of the base-excision DNA repair pathway. Base excision repair is initiated by DNA glycosylases, lesion-specific enzymes that intercept aberrant bases in DNA and catalyze their excision. How such proteins accomplish the feat of catalyzing no fewer than five sequential reaction steps using a single active site has been unknown. To help answer this, we report the structure of a trapped catalytic intermediate in DNA repair by human 8-oxoguanine DNA glycosylase. This structure and supporting biochemical results reveal that the enzyme sequesters the excised lesion base and exploits it as a cofactor to participate in catalysis. To our knowledge, the present example represents the first documented case of product-assisted catalysis in an enzyme-catalyzed reaction.


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Last modification of this entry: Oct. 6, 2010

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