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"In vivo regulation of the uvrA gene: role of the "-10" and "-35" promoter regions." |
Backendorf C, Brandsma JA, Kartasova T, van de Putte P |
Published Sept. 10, 1983 in Nucleic Acids Res volume 11 .
Pubmed ID: 6310514
Abstract:
| The effect of increasing deletions in the uvrA promoter region on the transcriptional efficiency was quantitatively analysed by fusion to the galK structural gene. A physical analysis of uvrA messenger RNA synthesis from the different deletion plasmids was performed using the S1 mapping technique. Both methods indicate that the uvrA "-10" promoter sequence is sufficient to trigger uvrA transcription. Although not essential, the "-35" region, which is overlapping with the LexA binding site, is shown to have an enhancing function, as the exposure of this region after SOS induction results in a 3- to 4-fold increase in uvrA transcription. A model is presented which accounts both for the observed basal and induced expression of the uvrA gene on a molecular level. |
This publication refers to following REPAIRtoire entries:
| Proteins |
Last modification of this entry: Oct. 6, 2010
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