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"Cloning and sequencing of the NES24 gene of Saccharomyces cerevisiae."

Yoshida M, Shimma Y, Uno I, Toh-e A



Published March 1, 1994 in Yeast volume 10 .

Pubmed ID: 8017106

Abstract:
We have cloned NES24 using a temperature-sensitive nes24-1 mutant as a host and sequenced a 3162 bp XhoI-EcoRI DNA fragment containing the NES24 gene. Computer analysis revealed that this segment contains a 1806 bp open reading frame which is needed for complementation of the nes24-1 mutation. We found SUP8 in the region upstream of the NES24 gene, placing the NES24 gene on chromosome XIII. A protein homology search indicated that NES24 encodes a new protein. The disruption of the NES24 gene resulted in temperature-sensitive growth. The sequence has been deposited in DDBJ/EmBL/GenBank data bases under Accession Number D15052.


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Last modification of this entry: Oct. 6, 2010

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