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"Construction of a umuDC operon substitution mutation in Escherichia coli."

Woodgate R



Published March 1, 1992 in Mutat Res volume 281 .

Pubmed ID: 1371846

Abstract:
Using a specialized transducing lambda phage, the umuDC operon of Escherichia coli was deleted and replaced with the chloramphenicol acetyltransferase gene. The delta (umuDC)595::cat mutation was subsequently transferred by generalized P1 transduction into a variety of genetic backgrounds. It is concluded that the UmuDC proteins, which are normally required for inducible mutagenesis, are not essential for cell survival.


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Last modification of this entry: Oct. 6, 2010

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