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"Five hundredfold overproduction of DNA ligase after induction of a hybrid lambda lysogen constructed in vitro." |
Panasenko SM, Cameron JR, Davis RW, Lehman IR |
Published April 8, 1977 in Science volume 196 .
Pubmed ID: 322281
Abstract:
| A lambda vector that contains the gene for Escherichia coli DNA ligase (lambdagt4-lop-11 lig+) has been modified to achieve overproduction of this enzyme. The third Eco RI site in the lambda chromosome has been altered by mutation, and the left-hand Eco RI fragment has been shortened. The new vector, lambdagt4-lop-11 lig+, forms a stable lysogen which, upon induction, produces a 100-fold increase in DNA ligase activity. Introduction of a phage mutation (S7) that prevents cell lysis results in an even greater increase (500-fold). |
This publication refers to following REPAIRtoire entries:
| Genes |
Last modification of this entry: Oct. 6, 2010
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