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"Isolation and preliminary characterization of Escherichia coli mutants deficient in exonuclease VII." |
Vales LD, Rabin BA, Chase JW |
Published Sept. 1, 1983 in J Bacteriol volume 155 .
Pubmed ID: 6350262
Abstract:
| Strains of Escherichia coli containing reduced levels of exonuclease VII activity due to mutations in the xseB gene have been isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Seven mutants of independent origin deficient in exonuclease VII activity were obtained. Four of these contained defects in xseA, a locus which has been previously identified, and three others contained mutations in a gene distinct from xseA, which we have designated xseB. Genetic mapping studies place the xseB locus between proC and dnaZ. Exonuclease VII purified from KLC835 (xseA+ xseB3) is more heat labile than enzyme purified from the parent strain PA610 (xse+), showing that xseB is a structural gene for exonuclease VII. The isolation of lambda transducing phage carrying xseA is also described. |
This publication refers to following REPAIRtoire entries:
| Genes |
Last modification of this entry: Oct. 13, 2010
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