Welcome! Click here to login or here to register.
 |
|||||||||||||||||||
|
|||||||||||||||||||
 |
|||||||||||||||||||
Sgs1p |
Protein FULL name:
Nucleolar DNA helicase of the RecQ family involved in genome integrity maintenance; regulates chromosome synapsis and meiotic joint molecule/crossover formation; similar to human BLM and WRN proteins implicated in Bloom and Werner syndromes
Sgs1p (Saccharomyces cerevisiae) is product of expression of SGS1 gene.
FUNCTION: Interacts with topoisomerases II and TOP3. Could create a deleterious topological substrate that TOP3 preferentially resolves. The TOP3-SGS1 protein complex may function as a eukaryotic reverse gyrase introducing positive supercoils into extrachromosomal ribosomal DNA rings. Together with topoisomerase II has a role in chromosomal segregation. Maintains rDNA structure where it has a role in re-starting stalled replication forks.
SUBUNIT: Heterodimer with TOP3. Forms a complex with TOP3 and RMI1. Forms a ternary complex with a MLH1-MLH3 heterodimer (MutLbeta) during meiosis. Interacts with TOP2.
INTERACTION: Q12083:MLH3; NbExp=1; IntAct=EBI-17059, EBI-31634; Q12306:SMT3; NbExp=1; IntAct=EBI-17059, EBI-17490; P13099:TOP3; NbExp=1; IntAct=EBI-17059, EBI-19365;
SUBCELLULAR LOCATION: Nucleus, nucleolus.
SIMILARITY: Belongs to the helicase family. RecQ subfamily.
SIMILARITY: Contains 1 helicase ATP-binding domain.
SIMILARITY: Contains 1 helicase C-terminal domain.
SIMILARITY: Contains 1 HRDC domain.
| NCBI GenPept GI number(s): |
6323844 |
| Species: | Saccharomyces cerevisiae |
Links to other databases:
| Database | ID | Link |
| Uniprot | P35187 |
P35187 |
| PFAM: | - |
P35187 (Link - using uniprot id) |
| InterPro: | - |
P35187 (Link - using uniprot id) |
| CATH: | - | - |
| SCOP: | - | - |
| PDB: | - | - |
Protein sequence:
|
MVTKPSHNLRREHKWLKETATLQEDKDFVFQAIQKHIANKRPKTNSPPTT PSKDECGPGTTNFITSIPASGPTNTATKQHEVMQTLSNDTEWLSYTATSN QYADVPMVDIPASTSVVSNPRTPNGSKTHNFNTFRPHMASSLVENDSSRN LGSRNNNKSVIDNSSIGKQLENDIKLEVIRLQGSLIMALKEQSKLLLQKC SIIESTSLSEDAKRLQLSRDIRPQLSNMSIRIDSLEKEIIKAKKDGMSKD QSKGRSQVSSQDDNIISSILPSPLEYNTSSRNSNLTSTTATTVTKALAIT GAKQNITNNTGKNSNNDSNNDDLIQVLDDEDDIDCDPPVILKEGAPHSPA FPHLHMTSEEQDELTRRRNMRSREPVNYRIPDRDDPFDYVMGKSLRDDYP DVEREEDELTMEAEDDAHSSYMTTRDEEKEENELLNQSDFDFVVNDDLDP TQDTDYHDNMDVSANIQESSQEGDTRSTITLSQNKNVQVILSSPTAQSVP SNGQNQIGVEHIDLLEDDLEKDAILDDSMSFSFGRQHMPMSHSDLELIDS EKENEDFEEDNNNNGIEYLSDSDLERFDEERENRTQVADIQELDNDLKII TERKLTGDNEHPPPSWSPKIKREKSSVSQKDEEDDFDDDFSLSDIVSKSN LSSKTNGPTYPWSDEVLYRLHEVFKLPGFRPNQLEAVNATLQGKDVFVLM PTGGGKSLCYQLPAVVKSGKTHGTTIVISPLISLMQDQVEHLLNKNIKAS MFSSRGTAEQRRQTFNLFINGLLDLVYISPEMISASEQCKRAISRLYADG KLARIVVDEAHCVSNWGHDFRPDYKELKFFKREYPDIPMIALTATASEQV RMDIIHNLELKEPVFLKQSFNRTNLYYEVNKKTKNTIFEICDAVKSRFKN QTGIIYCHSKKSCEQTSAQMQRNGIKCAYYHAGMEPDERLSVQKAWQADE IQVICATVAFGMGIDKPDVRFVYHFTVPRTLEGYYQETGRAGRDGNYSYC ITYFSFRDIRTMQTMIQKDKNLDRENKEKHLNKLQQVMAYCDNVTDCRRK LVLSYFNEDFDSKLCHKNCDNCRNSANVINEERDVTEPAKKIVKLVESIQ NERVTIIYCQDVFKGSRSSKIVQANHDTLEEHGIGKSMQKSEIERIFFHL ITIRVLQEYSIMNNSGFASSYVKVGPNAKKLLTGKMEIKMQFTISAPNSR PSTSSSFQANEDNIPVIAQKSTTIGGNVAANPPRFISAKEHLRSYTYGGS TMGSSHPITLKNTSDLRSTQELNNLRMTYERLRELSLNLGNRMVPPVGNF MPDSILKKMAAILPMNDSAFATLGTVEDKYRRRFKYFKATIADLSKKRSS EDHEKYDTILNDEFVNRAAASSNGIAQSTGTKSKFFGANLNEAKENEQII NQIRQSQLPKNTTSSKSGTRSISKSSKKSANGRRGFRNYRGHYRGRK |
Sgs1p (Saccharomyces cerevisiae) belongs to following protein families:
References:
| Title | Authors | Journal |
| The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase. | Gangloff S, McDonald JP, Bendixen C, Arthur L, Rothstein R | Mol Cell Biol Dec. 1, 1994 |
| Sgs1: a eukaryotic homolog of E. coli RecQ that interacts with topoisomerase II in vivo and is required for faithful chromosome segregation. | Watt PM, Louis EJ, Borts RH, Hickson ID | Cell April 21, 1995 |
| The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII. | Bowman S, Churcher C, Badcock K, Brown D, Chillingworth T, Connor R, Dedman K, Devlin K, Gentles S, Hamlin N, Hunt S, Jagels K, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Skelton J, Walsh S, Whitehead S, Barrell B | Nature May 1, 1997 |
| The three-dimensional structure of the HRDC domain and implications for the Werner and Bloom syndrome proteins. | Liu Z, Macias MJ, Bottomley MJ, Stier G, Linge JP, Nilges M, Bork P, Sattler M | Structure Dec. 15, 1999 |
| Supercomplex formation between Mlh1-Mlh3 and Sgs1-Top3 heterocomplexes in meiotic yeast cells. | Wang TF, Kung WM | Biochem Biophys Res Commun Aug. 1, 2002 |
| Role of SGS1 and SLX4 in maintaining rDNA structure in Saccharomyces cerevisiae. | Kaliraman V, Brill SJ | Curr Genet Sept. 1, 2002 |
| RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex. | Chang M, Bellaoui M, Zhang C, Desai R, Morozov P, Delgado-Cruzata L, Rothstein R, Freyer GA, Boone C, Brown GW | EMBO J June 1, 2005 |
| Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. | Mullen JR, Nallaseth FS, Lan YQ, Slagle CE, Brill SJ | Mol Cell Biol June 1, 2005 |
Last modification of this entry: Oct. 6, 2010.
Add your own comment!
There is no comment yet.
|
|
|
|