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Protein FULL name:
DNA polymerase III beta-subunit [Escherichia coli].
DnaN (Escherichia coli strain K-12 substr. MG1655) is product of expression of dnaN gene.
DnaN is involved in:
MMR in Escherichia coli strain K-12 substr. MG1655
FUNCTION: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP- independent) along duplex DNA.
CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
SUBUNIT: The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)-(gamma/tau)-delta,delta', psi,chi- beta. The beta chain is a homodimer when not associated with the other components.
INTERACTION: P06710:dnaX; NbExp=1; IntAct=EBI-542385, EBI-549140; P0A6F5:groL; NbExp=1; IntAct=EBI-542385, EBI-543750; P69931:hda; NbExp=1; IntAct=EBI-542385, EBI-545453; P28630:holA; NbExp=2; IntAct=EBI-542385, EBI-549153; P0AG07:rpe; NbExp=1; IntAct=EBI-542385, EBI-546020; P05845:tnsE (xeno); NbExp=2; IntAct=EBI-542385, EBI-2434514; P0A8E7:yajQ; NbExp=1; IntAct=EBI-542385, EBI-370752; P77395:ybbN; NbExp=1; IntAct=EBI-542385, EBI-545297; P0AFX0:yhbH; NbExp=1; IntAct=EBI-542385, EBI-561113; P0AC53:zwf; NbExp=1; IntAct=EBI-542385, EBI-555656;
SUBCELLULAR LOCATION: Cytoplasm.
This protein can be a part of a given complexes:
Links to other databases:
Last modification of this entry: Oct. 12, 2010.
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