Welcome! Click here to login or here to register.
Home
Proteins
DNA damage
Diseases
Homologs
Pathways
Keywords
Publications
Draw a picture
 
Search
 
Links
Help
Contact





Bujnicki Lab Homepage

DnaE

Protein FULL name:

DNA polymerase III holoenzyme, alpha subunit [Escherichia coli].


DnaE (Escherichia coli strain K-12 substr. MG1655) is product of expression of dnaE gene.


DnaE is involved in:

MMR in Escherichia coli strain K-12 substr. MG1655
     


Keywords:



FUNCTION: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase.

CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

SUBUNIT: The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different dnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi- beta[4].

INTERACTION: P03007:dnaQ; NbExp=3; IntAct=EBI-549111, EBI-549131; P30855:evgS; NbExp=1; IntAct=EBI-549111, EBI-546983; P77374:ynfE; NbExp=1; IntAct=EBI-549111, EBI-556186;

SUBCELLULAR LOCATION: Cytoplasm.

SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE subfamily.


This protein can be a part of a given complexes:
NCBI GenPept GI number(s): 146663
Species: Escherichia coli

Links to other databases:

Database ID Link
Uniprot P10443 P10443
PFAM: - P10443 (Link - using uniprot id)
InterPro: - P10443 (Link - using uniprot id)
CATH: - -
SCOP: - -
PDB: - -


Protein sequence:
MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDFTNLCGL
VKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLL
ISKAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSAL
VDECVAFYEEHFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATN
DVRFIDSSDFDAHEIRVAIHDGFTLDDPKRPRNYSPQQYMRSEEEMCELF
ADIPEALANTVEIAKRCNVTVRLGEYFLPQFPTGDMSTEDYLVKRAKEGL
EERLAFLFPDEEERLKRRPEYDERLETELQVINQMGFPGYFLIVMEFIQW
SKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFLNPERVSMP
DFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARK
LEGVTRNAGKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAG
LVKFDFLGLRTLTIINWALEMINKRRAKNGEPPLDIAAIPLDDKKSFDML
QRSETTAVFQLESRGMKDLIKRLQPDCFEDMIALVALFRPGPLQSGMVDN
FIDRKHGREEISYPDVQWQHESLKPVLEPTYGIILYQEQVMQIAQVLSGY
TLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAELAMKIFDLVEK
FAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVVGL
VDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAII
EARNKGGYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMN
SLGDALKAADQHAKAEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVL
DGERETLGLYLTGHPINQYLKEIERYVGGVRLKDMHPTERGKVITAAGLV
VAARVMVTKRGNRIGICTLDDRSGRLEVMLFTDALDKYQQLLEKDRILIV
SGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISLTDRQIDDQLLNR
LRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDLRGLI
GSEQVELEFD

References:

Title Authors Journal
Sequence analysis of the Escherichia coli dnaE gene. Tomasiewicz HG, McHenry CS J Bacteriol Dec. 1, 1987
Sequence analysis of the Escherichia coli dnaE gene. Tomasiewicz HG, McHenry CS J Bacteriol July 1, 1991
Accessory protein function in the DNA polymerase III holoenzyme from E. coli. O'Donnell M Bioessays Jan. 1, 1992
Growth rate regulation of Escherichia coli acetyl coenzyme A carboxylase, which catalyzes the first committed step of lipid biosynthesis. Li SJ, Cronan JE Jr J Bacteriol Feb. 1, 1993
Antimutator mutations in the alpha subunit of Escherichia coli DNA polymerase III: identification of the responsible mutations and alignment with other DNA polymerases. Fijalkowska IJ, Schaaper RM Genetics Aug. 1, 1993
The complete genome sequence of Escherichia coli K-12. Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y Science Sept. 5, 1997
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T Mol Syst Biol Jan. 1, 2006


Last modification of this entry: Oct. 15, 2010.

Add your own comment!

There is no comment yet.
Welcome stranger! Click here to login or here to register.
Valid HTML 4.01! This site is Emacs powered. Made with Django.