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"Differential roles of phosphorylation in the formation of transcriptional active RNA polymerase I."

Fath S, Milkereit P, Peyroche G, Riva M, Carles C, Tschochner H



Published Dec. 4, 2001 in Proc Natl Acad Sci U S A volume 98 .

Pubmed ID: 11717393

Abstract:
Regulation of rDNA transcription depends on the formation and dissociation of a functional complex between RNA polymerase I (pol I) and transcription initiation factor Rrn3p. We analyzed whether phosphorylation is involved in this molecular switch. Rrn3p is a phosphoprotein that is predominantly phosphorylated in vivo when it is not bound to pol I. In vitro, Rrn3p is able both to associate with pol I and to enter the transcription cycle in its nonphosphorylated form. By contrast, phosphorylation of pol I is required to form a stable pol I-Rrn3p complex for efficient transcription initiation. Furthermore, association of pol I with Rrn3p correlates with a change in the phosphorylation state of pol I in vivo. We suggest that phosphorylation at specific sites of pol I is a prerequisite for proper transcription initiation and that phosphorylation/dephosphorylation of pol I is one possibility to modulate cellular rDNA transcription activity.


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Last modification of this entry: Oct. 6, 2010

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