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Rad50p |
Rad50p is involved in:
NHEJ in Saccharomyces cerevisiae
- MRX complex joins the DNA/YKu70/80 complex in Saccharomyces cerevisiae
- Processing with polymerase 4 in Saccharomyces cerevisiae
- Gap filling and non-ligatable DNA ends processing in Saccharomyces cerevisiae
- Ligation of DNA strands by Dnl4/Lif1/Nej1 complex in Saccharomyces cerevisiae
Keywords:
FUNCTION: Involved in DNA double-strand break repair (DSBR). The rad50/mre11 complex possesses single-strand endonuclease activity and ATP-dependent double-strand-specific exonuclease activity. Rad50 provides ATP-dependent control of mre11 by unwinding and/or repositioning DNA ends into the mre11 active site.
COFACTOR: Binds 1 zinc ion per homodimer (By similarity).
SUBUNIT: Homodimer (By similarity). Forms a complex with MRE11.
INTERACTION: P25390:SSK22; NbExp=1; IntAct=EBI-14700, EBI-18129;
DOMAIN: The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc- hook of another RAD50 molecule, thereby forming a V-shaped homodimer (By similarity).
MISCELLANEOUS: Present with 830 molecules/cell in log phase SD medium.
SIMILARITY: Belongs to the SMC family. RAD50 subfamily.
SIMILARITY: Contains 1 zinc-hook domain.
| NCBI GenPept GI number(s): |
1255968 |
| Species: | Saccharomyces cerevisiae |
Links to other databases:
| Database | ID | Link |
| Uniprot | P12753 |
P12753 |
| PFAM: | - |
P12753 (Link - using uniprot id) |
| InterPro: |
IPR003395 IPR003439 IPR004584 IPR007517 IPR013134 |
IPR003395 IPR003439 IPR004584 IPR007517 IPR013134 |
| CATH: | - | - |
| SCOP: | - | - |
| PDB: | - | - |
Protein sequence:
|
MSAIYKLSIQGIRSFDSNDRETIEFGKPLTLIVGMNGSGKTTIIECLKYA TTGDLPPNSKGGVFIHDPKITGEKDIRAQVKLAFTSANGLNMIVTRNIQL LMKKTTTTFKTLEGQLVAINNSGDRSTLSTRSLELDAQVPLYLGVPKAIL EYVIFCHQEDSLWPLSEPSNLKKKFDEIFQAMKFTKALDNLKSIKKDMSV DIKLLKQSVEHLKLDKDRSKAMKLNIHQLQTKIDQYNEEVSEIESQLNEI TEKSDKLFKSNQDFQKILSKVENLKNTKLSISDQVKRLSNSIDILDLSKP DLQNLLANFSKVLMDKNNQLRDLETDISSLKDRQSSLQSLSNSLIRRQGE LEAGKETYEKNRNHLSSLKEAFQHKFQGLSNIENSDMAQVNHEMSQFKAF ISQDLTDTIDQFAKDIQLKETNLSDLIKSITVDSQNLEYNKKDRSKLIHD SEELAEKLKSFKSLSTQDSLNHELENLKTYKEKLQSWESENIIPKLNQKI EEKNNEMIILENQIEKFQDRIMKTNQQADLYAKLGLIKKSINTKLDELQK ITEKLQNDSRIRQVFPLTQEFQRADLEMDFQKLFINMQKNIAINNKKMHE LDRRYTNALYNLNTIEKDLQDNQKSKEKVIQLLSENLPEDCTIDEYNDVL EETELSYKTALENLKMHQTTLEFNRKALEIAERDSCCYLCSRKFENESFK SKLLQELKTKTDANFEKTLKDTVQNEKEYLHSLRLLEKHIITLNSINEKI DNSQKCLEKAKEETKTSKSKLDELEVDSTKLKDEKELAESEIRPLIEKFT YLEKELKDLENSSKTISEELSIYNTSEDGIQTVDELRDQQRKMNDSLREL RKTISDLQMEKDEKVRENSRMINLIKEKELTVSEIESSLTQKQNIDDSIR SKRENINDIDSRVKELEARIISLKNKKDEAQSVLDKVKNERDIQVRNKQK TVADINRLIDRFQTIYNEVVDFEAKGFDELQTTIKELELNKAQMLELKEQ LDLKSNEVNEEKRKLADSNNEEKNLKQNLELIELKSQLQHIESEISRLDV QNAEAERDKYQEESLRLRTRFEKLSSENAGKLGEMKQLQNQIDSLTHQLR TDYKDIEKNYHKEWVELQTRSFVTDDIDVYSKALDSAIMKYHGLKMQDIN RIIDELWKRTYSGTDIDTIKIRSDEVSSTVKGKSYNYRVVMYKQDVELDM RGRCSAGQKVLASIIIRLALSETFGANCGVIALDEPTTNLDEENIESLAK SLHNIINMRRHQKNFQLIVITHDEKFLGHMNAAAFTDHFFKVKRDDRQKS QIEWVDINRVTY |
References:
| Title | Authors | Journal |
| The yeast RAD50 gene encodes a predicted 153-kD protein containing a purine nucleotide-binding domain and two large heptad-repeat regions. | Alani E, Subbiah S, Kleckner N | Genetics May 1, 1989 |
| The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications. | Philippsen P, Kleine K, Pohlmann R, Dusterhoft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andre B, Baladron V, Ballesta JP, Becam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Hani J, et al. | Nature May 1, 1997 |
| Sequence analysis of the 33 kb long region between ORC5 and SUI1 from the left arm of chromosome XIV from Saccharomyces cerevisiae. | Sen-Gupta M, Guldener U, Beinhauer J, Fiedler T, Hegemann JH | Yeast July 1, 1997 |
| Global analysis of protein expression in yeast. | Ghaemmaghami S, Huh WK, Bower K, Howson RW, Belle A, Dephoure N, O'Shea EK, Weissman JS | Nature Oct. 16, 2003 |
| A multidimensional chromatography technology for in-depth phosphoproteome analysis. | Albuquerque CP, Smolka MB, Payne SH, Bafna V, Eng J, Zhou H | Mol Cell Proteomics July 1, 2008 |
Last modification of this entry: Oct. 15, 2010.
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