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Pol4p |
Protein FULL name:
DNA polymerase IV, undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta
Pol4p (Saccharomyces cerevisiae) is product of expression of POL4 gene.
Pol4p is involved in:
NHEJ in Saccharomyces cerevisiae
Keywords:
FUNCTION: Repair polymerase. Involved in gap-filling in DNA nonhomologous end joining (NHEJ) required for double-strand break repair. Seems to conduct DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Preferentially acts upon short gaps formed by the alignment of linear duplexes with complementary single-strand ends. Required for filling gaps that need removal of a 5'- or 3'- terminal mismatch, however lacks nuclease activities.
CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
COFACTOR: Magnesium (By similarity).
ENZYME REGULATION: Stimulated by the interaction with the DNL4- LIF1 complex.
SUBUNIT: Interacts with DNL4 subunit of the DNL4-LIF1 complex.
INTERACTION: P53879:RHO5; NbExp=1; IntAct=EBI-6115, EBI-29054; P09959:SWI6; NbExp=1; IntAct=EBI-6115, EBI-18641;
SUBCELLULAR LOCATION: Nucleus.
INDUCTION: During meiosis.
SIMILARITY: Belongs to the DNA polymerase type-X family.
| NCBI GenPept GI number(s): |
10383782 |
| Species: | Saccharomyces cerevisiae |
Links to other databases:
| Database | ID | Link |
| Uniprot | P25615 |
P25615 |
| PFAM: | - |
P25615 (Link - using uniprot id) |
| InterPro: | - |
P25615 (Link - using uniprot id) |
| CATH: | - | - |
| SCOP: | - | - |
| PDB: | - | - |
Protein sequence:
|
MSLKGKFFAFLPNPNTSSNKFFKSILEKKGATIVSSIQNCLQSSRKEVVI LIEDSFVDSDMHLTQKDIFQREAGLNDVDEFLGKIEQSGIQCVKTSCITK WVQNDKFAFQKDDLIKFQPSIIVISDNADDGQSSTDKESEISTDVESERN DDSNNKDMIQASKPLKRLLQGDKGRASLVTDKTKYKNNELIIGALKRLTK KYEIEGEKFRARSYRLAKQSMENCDFNVRSGEEAHTKLRNIGPSIAKKIQ VILDTGVLPGLNDSVGLEDKLKYFKNCYGIGSEIAKRWNLLNFESFCVAA KKDPEEFVSDWTILFGWSYYDDWLCKMSRNECFTHLKKVQKALRGIDPEC QVELQGSYNRGYSKCGDIDLLFFKPFCNDTTELAKIMETLCIKLYKDGYI HCFLQLTPNLEKLFLKRIVERFRTAKIVGYGERKRWYSSEIIKKFFMGVK LSPRELEELKEMKNDEGTLLIEEEEEEETKLKPIDQYMSLNAKDGNYCRR LDFFCCKWDELGAGRIHYTGSKEYNRWIRILAAQKGFKLTQHGLFRNNIL LESFNERRIFELLNLKYAEPEHRNIEWEKKTA |
Pol4p (Saccharomyces cerevisiae) is able to recognize following damages:
Pol4p (Saccharomyces cerevisiae) belongs to following protein families:
References:
Last modification of this entry: Oct. 15, 2010.
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