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Protein FULL name:
DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) [Escherichia coli str. K-12 substr. MG1655].
Nth (Escherichia coli strain K-12 substr. MG1655) is product of expression of nth gene.
Nth is involved in:
BER in Escherichia coli strain K-12 substr. MG1655
FUNCTION: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
COFACTOR: Binds 1 4Fe-4S cluster. The cluster is not important for the catalytic activity, but which is probably involved in the proper positioning of the enzyme along the DNA strand.
INTERACTION: P0AFG8:aceE; NbExp=1; IntAct=EBI-555213, EBI-542683;
SIMILARITY: Belongs to the nth/mutY family.
Links to other databases:
Nth (Escherichia coli strain K-12 substr. MG1655) is able to recognize following damages:
Last modification of this entry: Oct. 14, 2010.
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