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PolI

Protein FULL name:

DNA polymerase I [Escherichia coli]

PolI (Escherichia coli strain K-12 substr. MG1655) is product of expression of polA gene.

PolI is involved in:

HRR in Escherichia coli strain K-12 substr. MG1655

BER in Escherichia coli strain K-12 substr. MG1655

NER in Escherichia coli strain K-12 substr. MG1655

Keywords:

FUNCTION: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.

CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

SUBUNIT: Single-chain monomer with multiple functions.

INTERACTION: P0A9G4:cueR; NbExp=1; IntAct=EBI-552540, EBI-1117686;

SIMILARITY: Belongs to the DNA polymerase type-A family.

SIMILARITY: Contains 1 3'-5' exonuclease domain.

SIMILARITY: Contains 1 5'-3' exonuclease domain.

WEB RESOURCE: Name=Worthington enzyme manual; [LINK]

NCBI GenPept GI number(s):	147312 42461
Species:	Escherichia coli

Links to other databases:

Database	ID	Link
Uniprot	P00582	P00582
PFAM:	-	P00582 (Link - using uniprot id)
InterPro:	IPR001098 IPR002298 IPR002421 IPR002562 IPR008918 IPR012337	IPR001098 IPR002298 IPR002421 IPR002562 IPR008918 IPR012337
CATH:	-	-
SCOP:	-	-
PDB:	-	-

Protein sequence:

MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLNMLRSLI
MQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVK
AMGLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNIT
LINTMTNTILGPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKT
AQALLQGLGGLDTLYAEPEKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLA
TIKTDVELELTCEQLEVQQPAAEELLGLFKKYEFKRWTADVEAGKWLQAK
GAKPAAKPQETSVADEAPEVTATVISYDNYVTILDEETLKAWIAKLEKAP
VFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDAPDQISRER
ALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAE
DADVTLQLHLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPK
VLHNHSEELTLRLAELEKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLK
KTPGGAPSTSEEVLEELALDYPLPKVILEYRGLAKLKSTYTDKLPLMINP
KTGRVHTSYHQAVTATGRLSSTDPNLQNIPVRNEEGRRIRQAFIAPEDYV
IVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAEVFGLPLETVT
SEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGVLE
YMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQG
TAADIIKRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQI
HQLMENCTRLDVPLLVEVGSGENWDQAH

References:

Title	Authors	Journal
Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I.	Joyce CM, Kelley WS, Grindley ND	J Biol Chem Jan. 25, 1982
Escherichia coli DNA polymerase I. Sequence characterization and secondary structure prediction.	Brown WE, Stump KH, Kelley WS	J Biol Chem Jan. 25, 1982
Identification of two genes immediately downstream from the polA gene of Escherichia coli.	Joyce CM, Grindley ND	J Bacteriol Dec. 1, 1982
Genetic characterization of early amber mutations in the Escherichia coli polA gene and purification of the amber peptides.	Kelley WS, Joyce CM	J Mol Biol March 15, 1983
Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP.	Ollis DL, Brick P, Hamlin R, Xuong NG, Steitz TA	Nature Jan. 1, 1985
Structural basis for the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I: a two metal ion mechanism.	Beese LS, Steitz TA	EMBO J Feb. 1, 1991
Sequential proton NMR resonance assignments, circular dichroism, and structural properties of a 50-residue substrate-binding peptide from DNA polymerase I.	Mullen GP, Vaughn JB Jr, Mildvan AS	Arch Biochem Biophys Jan. 15, 1993
Structure of DNA polymerase I Klenow fragment bound to duplex DNA.	Beese LS, Derbyshire V, Steitz TA	Science April 16, 1993
Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes.	Plunkett G 3rd, Burland V, Daniels DL, Blattner FR	Nucleic Acids Res July 25, 1993
Crystal structures of the Klenow fragment of DNA polymerase I complexed with deoxynucleoside triphosphate and pyrophosphate.	Beese LS, Friedman JM, Steitz TA	Biochemistry Dec. 28, 1993
The complete genome sequence of Escherichia coli K-12.	Blattner FR, Plunkett G 3rd, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y	Science Sept. 5, 1997
Structural principles for the inhibition of the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I by phosphorothioates.	Brautigam CA, Steitz TA	J Mol Biol March 27, 1998
Structures of normal single-stranded DNA and deoxyribo-3'-S-phosphorothiolates bound to the 3'-5' exonucleolytic active site of DNA polymerase I from Escherichia coli.	Brautigam CA, Sun S, Piccirilli JA, Steitz TA	Biochemistry Feb. 12, 1999
Structural origins of the exonuclease resistance of a zwitterionic RNA.	Teplova M, Wallace ST, Tereshko V, Minasov G, Symons AM, Cook PD, Manoharan M, Egli M	Proc Natl Acad Sci U S A Dec. 7, 1999
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.	Hayashi K, Morooka N, Yamamoto Y, Fujita K, Isono K, Choi S, Ohtsubo E, Baba T, Wanner BL, Mori H, Horiuchi T	Mol Syst Biol Jan. 1, 2006

Last modification of this entry: Oct. 19, 2010.

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