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POLE |
Protein FULL name:
DNA polymerase epsilon catalytic subunit A [Homo sapiens].
POLE (Homo sapiens) is product of expression of POLE gene.
POLE is involved in:
NER in Homo sapiens BER in Homo sapiens
- Strand scission by APEX/PCNA/FEN1/PARP/pol beta/pol epsilon/lig1 complex in Homo sapiens
- Gap filling and strand displacement with polymerase epsilon in Homo sapiens
- Removing overhang by complex with pol epsilon in Homo sapiens
Keywords:
- mismatch repair (MMR)
- transcription-coupled repair NER (TCR-NER)
- nucleotide excision repair (NER)
- base excision repair (BER)
- long path BER (LP-BER)
- global genome repair NER (GGR-NER)
- DNA polymerase
FUNCTION: Participates in DNA repair and in chromosomal DNA replication.
CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
SUBUNIT: Consists of two subunits (258 kDa and 55 kDa). Interacts with RAD17 and TOPBP1.
INTERACTION: P49842:STK19; NbExp=1; IntAct=EBI-348526, EBI-347581;
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: The DNA polymerase activity domain resides in the N- terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C. The C-terminus may also regulate the catalytic activities of the enzyme.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the DNA polymerase type-B family.
SEQUENCE CAUTION: Sequence=AAA15448.1; Type=Frameshift; Positions=443, 448; Sequence=AAA15448.1; Type=Miscellaneous discrepancy;
WEB RESOURCE: Name=NIEHS-SNPs; [LINK]
| NCBI GenPept GI number(s): |
62198237 116241339 |
| Species: | Homo sapiens |
Links to other databases:
| Database | ID | Link |
| Uniprot | Q07864 |
Q07864 |
| PFAM: | - |
Q07864 (Link - using uniprot id) |
| InterPro: | - |
Q07864 (Link - using uniprot id) |
| CATH: | - | - |
| SCOP: | - | - |
| PDB: | - | - |
Protein sequence:
|
MSLRSGGRRRADPGADGEASRDDGATSSVSALKRLERSQWTDKMDLRFGF ERLKEPGEKTGWLINMHPTEILDEDKRLGSAVDYYFIQDDGSRFKVALPY KPYFYIATRKGCEREVSSFLSKKFQGKIAKVETVPKEDLDLPNHLVGLKR NYIRLSFHTVEDLVKVRKEISPAVKKNREQDHASDAYTALLSSVLQRGGV ITDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHVAHWYNVRYRG NAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMI DGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEH VQETKPTIMVTYNGDFFDWPFVEARAAVHGLSMQQEIGFQKDSQGEYKAP QCIHMDCLRWVKRDSYLPVGSHNLKAAAKAKLGYDPVELDPEDMCRMATE QPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPDEVLRKGSGTL CEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDSETYVGGHVEALESG VFRSDIPCRFRMNPAAFDFLLQRVEKTLRHALEEEEKVPVEQVTNFEEVC DEIKSKLASLKDVPSRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEAT CAACDFNKPGANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFP EGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHITKVEERLTTICQRE NSFYVDTVRAFRDRRYEFKGLHKVWKKKLSAAVEVGDAAEVKRCKNMEVL YDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGANIITQARELI EQIGRPLELDTDGIWCVLPNSFPENFVFKTTNVKKPKVTISYPGAMLNIM VKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGK KLKKRYAVFNEDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLE EVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQK STSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQ AEPTVRKHFLRKWLKSSSLQDFDIRAILDWDYYIERLGSAIQKIITIPAA LQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLEGRRQVTMAEA SEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVP WQEILGQPPALGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEG VLRPGAIRDGPATGLGSFLRRTARSILDLPWQIVQISETSQAGLFRLWAL VGSDLHCIRLSIPRVFYVNQRVAKAEEGASYRKVNRVLPRSNMVYNLYEY SVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVHLGCVCVVNKQ LVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHAQAHKALF GIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLPPPK HTFEVRAETDLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEI PVLEEFPLVPICVADKINYGVLDWQRHGARRMIRHYLNLDTCLSQAFEMS RYFHIPIGNLPEDISTFGSDLFFARHLQRHNHLLWLSPTARPDLGGKEAD DNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTILQSHHVNDMEG ADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRILKSMVV GWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMKKLFL QLIAEFKRLGSSVIYANFNRIILCTKKRRVEDAIAYVEYITSSIHSKETF HSLTISFSRCWEFLLWMDPSNYGGIKGKVSSRIHCGLQDSQKAGGAEDEQ ENEDDEEERDGEEEEEAEESNVEDLLENNWNILQFLPQAASCQNYFLMIV SAYIVAVYHCMKDGLRRSAPGSTPVRRRGASQLSQEAEGAVGALPGMITF SQDYVANELTQSFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNP ALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRS YVLPEVICRSCNFCRDLDLCKDSSFSEDGAVLPQWLCSNCQAPYDSSAIE MTLVEVLQKKLMAFTLQDLVCLKCRGVKETSMPVYCSCAGDFALTIHTQV FMEQIGIFRNIAQHYGMSYLLETLEWLLQKNPQLGH |
POLE (Homo sapiens) is able to recognize following damages:
POLE (Homo sapiens) belongs to following protein families:
References:
| Title | Authors | Journal |
| Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase epsilon. | Kesti T, Frantti H, Syvaoja JE | J Biol Chem May 15, 1993 |
| BRCT domain-containing protein TopBP1 functions in DNA replication and damage response. | Makiniemi M, Hillukkala T, Tuusa J, Reini K, Vaara M, Huang D, Pospiech H, Majuri I, Westerling T, Makela TP, Syvaoja JE | J Biol Chem Aug. 10, 2001 |
| The human checkpoint Rad protein Rad17 is chromatin-associated throughout the cell cycle, localizes to DNA replication sites, and interacts with DNA polymerase epsilon. | Post SM, Tomkinson AE, Lee EY | Nucleic Acids Res Oct. 1, 2003 |
| ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. | Matsuoka S, Ballif BA, Smogorzewska A, McDonald ER 3rd, Hurov KE, Luo J, Bakalarski CE, Zhao Z, Solimini N, Lerenthal Y, Shiloh Y, Gygi SP, Elledge SJ | Science May 25, 2007 |
Last modification of this entry: Oct. 19, 2010.
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